Myofibrillogenesis in living cells microinjected with fluorescently labeled alpha-actinin.
نویسندگان
چکیده
منابع مشابه
Myofibrillogenesis in living cells microinjected with fluorescently labeled alpha-actinin
Fluorescently labeled alpha-actinin, isolated from chicken gizzards, breast muscle, or calf brains, was microinjected into cultured embryonic myotubes and cardiac myocytes where it was incorporated into the Z-bands of myofibrils. The localization in injected, living cells was confirmed by reacting permeabilized myotubes and cardiac myocytes with fluorescent alpha-actinin. Both living and permea...
متن کاملFormation and alignment of Z lines in living chick myotubes microinjected with rhodamine-labeled alpha-actinin
We have used fluorescence analogue cytochemistry in conjunction with time lapse recording to study the dynamics of alpha-actinin, a major component of the Z line, during myofibrillogenesis. Rhodamine-labeled alpha-actinin microinjected into living cultured chick skeletal myotubes became localized in discrete cellular structures within 1 h and remained specifically associated with structures for...
متن کاملDistribution of Fluorescently Labeled a-Actinin in Living and Fixed Fibroblasts
The distribution of fluorescently labeled a-actinin after microinjection into fibroblasts has been determined in both living and fixed cells. We have found that the distribution of the injected tetramethylrhodamine isothiocyanate-labeled protein (TMRITC-a-actinin) in living cells, which is in ruffling membranes, actin microfilament bundles, and polygonal microfilament networks (Feramisco, 1979,...
متن کاملObservations of microfilament bundles in living cells microinjected with fluorescently labelled contractile proteins.
Fluorescently labelled contractile proteins (alpha-actinin and filamin) were used to study the dynamic nature of three types of microfilament bundles: myofibrils, stress fibres and polygonal networks. Cultured muscle and non-muscle cells that were microinjected with fluorescent alpha-actinin rapidly incorporated the labelled protein into Z-bands, stress fibre densities and the polygonal foci. L...
متن کاملMolecular cytochemistry: incorporation of fluorescently labeled actin into living cells.
Actin labeled with 5-iodoacetamidofluorescein has been incorporated into the functional pool of actin in Chaos carolinensis and Physarum polycephalum by direct microinjection. The functional activity of the labeled actin has been analyzed at three levels of organization as: (a) with the purified actin, (b) in motile extracts of cells, and (c) in living motile cells. The labeled actin exhibited ...
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ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 1986
ISSN: 0021-9525,1540-8140
DOI: 10.1083/jcb.102.6.2053